Bioinformatic and empirical analysis of a gene encoding serine/threonine protein kinase regulated in response to chemical and biological fertilizers in two maize (Zea mays L.) cultivars

Authors

  • Abbas Alemzadeh Department of Crop Production and Plant Breeding, School of Agriculture, Shiraz University, Shiraz, Iran
  • Ida Azad Department of Crop Production and Plant Breeding, School of Agriculture, Shiraz University, Shiraz, Iran | Plant Breeding and Biotechnology Department, Agricultural Sciences and Natural Resources University of Sari, Sari, Iran
Abstract:

Molecular structure of a gene, ZmSTPK1, encoding a serine/threonine protein kinase in maize was analyzed by bioinformatic tool and its expression pattern was studied under chemical biological fertilizers. Bioinformatic analysis cleared that ZmSTPK1 is located on chromosome 10, from position 141015332 to 141017582. The full genomic sequence of the gene is 2251 bp in length and includes 2 exons. Its cDNA length is 1900 bp with a 5'-untranslated region of 311 bp and 3'-untranslated region of 341 bp, of which 1248 bp from open reading frame encoding 415 amino acid residues with a molecular weight of 46 kDa and an isoelectric point 7.2. Also, an upstream open reading frame contains 100 aa was found at -12 position from ATG initiation codon. ZmSTPK1 with a long half-life, 10 hours in Escherichia coli, and instability index of 32.25 is classified as a stable protein. A calmodulin binding domain was found in ZmSTPK1 at position from 395 to 405 in C-terminal end. The helical wheel analysis showed that the stretch of residues Ile-395 to Asp-415 has the potential to form a charged amphiphilic a-helix characteristic of a calmodulin-binding region. Two P1BS-like motifs, which are present in the promoter regions of Pi starvation-induced genes, were located at positions -48 and -867 from ATG initiation codon. The expression of ZmSTPK1 responded to available phosphate, and its expression up-regulated under phosphate starvation

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Assessment Maize Hybrids Response to Biological and Chemical Nitrogen Fertilizers

This research set out to evaluate the effect of combined biological and chemical nitrogen fertilizers on some corn hybrids. To this end an experiment was conducted as split plot on the basis of randomized complete block design with four replications. Main plots were included: use of chemical and biological fertilizers in four levels: 100% chemical fertilizer, 75% chemical fertilizer + 100% bio-...

full text

Maize (Zea mays L.).

Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation proced...

full text

a contrastive analysis of concord and head parameter in english and azerbaijani

این پایان نامه به بررسی و مقایسه دو موضوع مطابقه میان فعل و فاعل (از نظر شخص و مشار) و هسته عبارت در دو زبان انگلیسی و آذربایجانی می پردازد. اول رابطه دستوری مطابقه مورد بررسی قرار می گیرد. مطابقه به این معناست که فعل مفرد به همراه فاعل مفرد و فعل جمع به همراه فاعل جمع می آید. در انگلیسی تمام افعال، بجز فعل بودن (to be) از نظر شمار با فاعلشان فقط در سوم شخص مفرد و در زمان حال مطابقت نشان میدهند...

15 صفحه اول

Evaluation Effectiveness of Chemical and Biological Fertilizers Combination on Corn (Zea mays L.) Yield

In order to investigate the appropriate combination of chemical and bio-fertilizers and their effectiveness on corn seed yield while defoliating the upper leaves of the ear, a randomized complete block design experiment with four replications. Fertilizer treatments were applied at four levels including 100% urea, 75% urea + 100% Nitroxin, 50% urea + 100% Nitroxin, 25% urea + 100% Nitroxin. In h...

full text

Growth and Yield Response of Maize (Zea mays L.) to Different Rates of Nitrogen and Phosphorus Fertilizers in Southern Nigeria

The effect of twelve different rates of nitrogen and phosphorus fertilizers on growth and yield of maize (Zea mays L.) in southern Nigeria was evaluated between June and October, 2007. The results of the study showed that application of 120kgN/ha + 0kgP/ha and 60kgN/ha + 40kgP/ha significantly increased the growth of maize than other treatments. The application rate of 120kgN/ha +40kgP/ha signi...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 6  issue 2

pages  65- 75

publication date 2017-06-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023